Miroculus, Inc., a developer of revolutionary tools for personal lab automation today announced new research optimizing an automated PCR-free Whole Genome Sequencing (WGS) library prep workflow for very low input human samples. The work done in collaboration with the University of California San Francisco (UCSF) and Purigen Biosystems will be presented at the Advances in Genome Biology and Technology (AGBT) virtual conference March 1-3, 2021.
The collaboration demonstrated a hands-off sample preparation from biological specimens through library preparation using samples with as few as 2,500 cells, without the use of PCR. The powerful combination of personal automation with the IonicⓇ Purification System for DNA extraction from Purigen and the Miro Canvas™ from Miroculus for NGS library preparation provided superior sequencing results compared to manual efforts. This enabled successful detection of pathogenic variants while avoiding amplification-related artifacts.